FIGURE

Fig. 8

ID

ZDB-FIG-160517-25

Publication

Hsu et al., 2015 - A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.

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Fig. 8

Anterior myoD expression in somites is partially rescued by foxc1a overexpression at 16 hpf.Representative WISH results of (A) non-injected control mibⁿⁿ²⁰⁰² mutants, (B-E) 20 pg foxc1a mRNA injected mibⁿⁿ²⁰⁰² mutants and (F-J) 20 pg foxc1a mRNA injected WT (siblings) are shown. The rescued somites are marked by arrowheads. myoD expression in the anterior somites of E was rescued symmetrically, while those of B-D were rescued asymmetrically in terms of myoD expression pattern, which may be caused by the unequal segregation of injected foxc1a mRNA. Lower panel shows the genotyping results of the embryos shown in B-J. The genomic fragments of mib 5′ (amplified by tfi91 primer set), mib 3′ (amplified by ta52b primer set) and foxc1a were not detected in B-E, while they were amplified in F-J. fih and vhl were amplified in all samples except for the negative control N. None of the genomic fragments was detected in the negative control.

Expression Data

Gene:	foxc1a
Fish:	WT Df(Chr2:mib1,foxc1a,rab18b,yme1l1b,gata6,cables1,foxc1a,cldn1,znfl2a,dvl3a,selenot1a))nn2002/nn2002
Condition:	cold exposure
Anatomical Term:	somite
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	Df(Chr2:mib1,foxc1a,rab18b,yme1l1b,gata6,cables1,foxc1a,cldn1,znfl2a,dvl3a,selenot1a))nn2002/nn2002
Condition:	cold exposure
Observed In:	somite
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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