FIGURE

Fig. 2

ID

ZDB-FIG-160309-9

Publication

Tang et al., 2016 - Imaging tumour cell heterogeneity following cell transplantation into optically clear immune-deficient zebrafish

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Fig. 2

Imaging neovascularization in rag2^E450fs (casper) zebrafish engrafted with fluorescently labelled melanoma and ERMS.

(a) GFP-labelled, amelanotic melanoma implanted into rag2^E450fs (casper) fish (n=8 animals) and imaged following intravascular injection of crimson quantum dots (Qtracker 655). Whole animal images to the left and confocal images to the right ( × 100 magnification, 100-200 µm z-stack). Quantum dot fluorescence has been pseudo-coloured white. (b) GFP-labelled, amelanotic melanoma implanted into flk1:mCherry; rag2^E450fs (casper) transgenic zebrafish (n=4 animals). (c-d) GFP-labelled ERMS engrafted into flk1:mCherry; rag2^E450fs (casper) transgenic zebrafish (n=5 animals) and serially imaged over time (c, 13 d.p.t. and d, 21 d.p.t.). White arrowheads denote the site of intra-muscular injection of tumour cells. Scale bars equal 5 mm in whole animal images and 200 µm in confocal images.

Expression Data

Gene:	mCherry
Fish:	mitfa^w2/w2; rag2^fb101/fb101; mpv17^a9/a9; is5Tg
Condition:	cancer xenotransplantation
Anatomical Term:	vasculature
Stage:	Days 21-29

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; rag2^fb101/fb101; mpv17^a9/a9 mitfa^w2/w2; rag2^fb101/fb101; mpv17^a9/a9; is5Tg
Condition:	cancer xenotransplantation
Observed In:	whole organism whole organism embryonal rhabdomyosarcoma whole organism melanoma
Stage:	Adult

Phenotype Detail

Acknowledgments

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