FIGURE

Fig. 1

ID

ZDB-FIG-160309-8

Publication

Tang et al., 2016 - Imaging tumour cell heterogeneity following cell transplantation into optically clear immune-deficient zebrafish

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Fig. 1

Zebrafish cancers engraft into homozygous rag2^E450fs (casper) animals.

Donor animals shown in the left two panels while transplant recipients are to the right. (a) ZsYellow-labelled Myc-driven T-ALL from the syngeneic CG1 background, (b) EGFP-labelled neuroblastoma from AB background, (c) mCherry-labelled kRAS^G12D-driven ERMS from CG1 background, and (d) BRAF^V600E-induced melanoma arising in tp53^-/-nacre background. Tumour cells were transplanted intra-peritoneally (a,b,d) or intra-muscularly (c) into both rag2^E450fs (AB) and rag2^E450fs (casper)-recipient fish. Merged brightfield and fluorescent images are shown at 30 d.p.t. Cytospins of leukaemia cells are shown in a, whereas haematoxylin and eosin (H&E)-stained sections are shown in b-d. Scale bars equal 5 mm in whole animals images, 20 µm for cytospins shown in a, and 50 µm for histology sections shown in b-d.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rag2^fb101/fb101 (AB) mitfa^w2/w2; rag2^fb101/fb101; mpv17^a9/a9
Condition:	cancer xenotransplantation
Observed In:	whole organism whole organism embryonal rhabdomyosarcoma whole organism Lymphoid neoplasms whole organism melanoma whole organism neuroblastoma
Stage:	Adult

Phenotype Detail

Acknowledgments

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