FIGURE

Fig. 3

ID

ZDB-FIG-160309-10

Publication

Tang et al., 2016 - Imaging tumour cell heterogeneity following cell transplantation into optically clear immune-deficient zebrafish

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Fig. 3

Resolving tumour cell heterogeneity in ERMS at single-cell resolution following engraftment into flk1:mCherry; rag2^E450fs (casper) zebrafish.

(a) Epi-fluorescent images of primary ERMS in a 32-day-old myf5:GFP; myogenin-H2b:mRFP; mylpfa:lyn-cyan triple transgenic zebrafish. (b) Epi-fluorescent images of flk1:mCherry; rag2^E450fs (casper)-recipient fish engrafted intra-muscularly with fluorescently labelled ERMS at 28 d.p.t. (n=4 animals). (c) Confocal image with mCherry-labelled vasculature outlined by white dashed lines (left, × 100 magnification). Higher magnification of boxed region (right, × 400 magnification). Myosin-expressing, differentiated cells (Diff.) and less frequent myf5-GFP+ tumour-propagating cell (TPC) denoted by arrowheads. Scale bar equals 2 mm in a, 5 mm in b, 100 µm (c, left panel) and 25 µm (c, right panel).

Expression Data

Gene:	mCherry
Fish:	mitfa^w2/w2; rag2^fb101/fb101; mpv17^a9/a9; is5Tg
Condition:	cancer xenotransplantation
Anatomical Term:	vasculature
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; rag2^fb101/fb101; mpv17^a9/a9; is5Tg
Condition:	cancer xenotransplantation
Observed In:	whole organism whole organism embryonal rhabdomyosarcoma
Stage:	Adult

Phenotype Detail

Acknowledgments

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