FIGURE

Fig. 5

ID

ZDB-FIG-160129-8

Publication

Zheng et al., 2015 - Caffeine reduces hepatic lipid accumulation through regulation of lipogenesis and ER stress in zebrafish larvae

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Fig. 5

Effects of caffeine on the expression of selected genes in livers of zebrafish larvae overfed for 20 days. The relative mRNA expression of genes involved in lipid metabolism (a), ER stress (b), inflammatory factors (c), and autophage (d) in control (30 mg/d) and 5 % caffeine group compared with gene expression in model group (180 mg/d) by qRT-PCR. Expression analysis of the selected genes using cDNA prepared from liver of zebrafish larvae (n = 20) in each group. Data is expressed in mean ± SEM, n = 5. Protein expression of ER stress (e) and inflammatory factor (f) was examined by western blot in caffeine treatment and model group zebrafish larvae. * P < 0.05, * *P < 0.01, * * *P < 0.001 by one-way ANOVA

Expression Data

Genes:	acaca acox3 atf6 atg3 atg12 becn1 cd36 cpt1aa ddit3 eif2ak3 ern2 fasn hspa5 il1b nkap pparab pparg srebf1 tnfa ucp2
Antibodies:	Ab2-ddit3 Ab2-il1b Ab5-hspa5
Fish:	WT
Conditions:	unlimited food availability chemical treatment: caffeine, unlimited food availability
Anatomical Term:	whole organism
Stage:	Days 14-20

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Conditions:	unlimited food availability chemical treatment: caffeine, unlimited food availability
Observed In:	whole organism
Stage:	Days 14-20

Phenotype Detail

Acknowledgments

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