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Fig. 5

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ZDB-FIG-151113-4

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Xu et al., 2015 - Temporal-Spatial Resolution Fate Mapping Reveals Distinct Origins for Embryonic and Adult Microglia in Zebrafish

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Fig. 5

The RBI- but not VDA-Region-Derived Microglia Are Depleted in pu.1^G242D Mutants

(A) Microglia are largely absent in pu.1^G242D mutants at 3 dpf, but are fully recovered at 6 dpf. Stacked confocal images of immunohistochemistry staining of Lcp1 of 3-dpf, 4-dpf, 5-dpf, and 6-dpf WT and pu.1^G242D embryos from the dorsal view of the brain shows the recovery of microglia in pu.1^G242D mutants from 5 dpf onward.

(B) Quantification of Lcp1⁺ microglia number in WT (n = 6) and pu.1^G242D embryos (n = 6) at 3 dpf and 6 dpf. Error bars represent mean ± SEM. p < 0.001.

(C) Coro1a-GFP⁺ microglia are observed in VDA- but not RBI-region-labeled pu.1^G242D embryos at 8 dpf. White arrows indicate the coro1a-GFP⁺ microglia in the brain (dorsal view, rostral to the left, a part of the optic tectum).

(D) Quantification of the contribution of RBI- and VDA-region-derived microglia in 8dpf pu.1^G242D embryos. For the RBI- and VDA-region-labeled embryos, n = 3 and 6, respectively. Error bars represent mean ± SEM. p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 34, Xu, J., Zhu, L., He, S., Wu, Y., Jin, W., Yu, T., Qu, J.Y., Wen, Z., Temporal-Spatial Resolution Fate Mapping Reveals Distinct Origins for Embryonic and Adult Microglia in Zebrafish, 632-641, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell