FIGURE

Fig. 1

ID

ZDB-FIG-150528-2

Publication

Liu et al., 2015 - DNA methyltransferase 1 functions through C/ebpa to maintain hematopoietic stem and progenitor cells in zebrafish

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Fig. 1

Impairment of definitive hematopoiesis in dnmt1 mutant zebrafish. (A-J) WISH analysis of cmyb expression from 36 hpf to 5 dpf. In ldd794 mutant, cmyb expression was decreased from 36 hpf (A-B) and absent at 5 dpf (I-J). (K, K′, L, L′) Expression of hematopoietic progenitor marker runx1 was decreased at 36 hpf. (K′, L′) Magnified images of the boxed regions in K and L, respectively. (M-N) Expression of hematopoietic progenitor marker scl was decreased at 5 dpf. (O-Z) WISH analysis of key hematopoietic markers in dnmt1 mutant embryos and wild-type siblings. Expression of erythrocyte progenitor marker gata1 (O-P), mature erythrocyte marker hbae1(Q, R), myeloid-specific marker mpx (S, T), macrophage marker lyz (U, V), l-plastin (W, X) were decreased. Expression of lymphocyte maker rag1 (Y, Y′, Z, Z′) was completely absent at 4 dpf. Blue arrows indicate the position of the thymus; red arrows indicate the CHT; black arrows indicate the AGM.

Expression Data

Genes:	gata1a hbae1.1 lcp1 lyz mpx myb rag1 runx1 tal1
Fish:	TU dnmt1^rj794/rj794 (TU)
Anatomical Terms:	hematopoietic stem cell myeloid cell nucleate erythrocyte thymus lymphocyte
Stage Range:	Prim-25 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnmt1^rj794/rj794 (TU)
Observed In:	definitive hemopoiesis hematopoietic stem cell myeloid cell nucleate erythrocyte thymus lymphocyte
Stage Range:	Prim-25 to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Hematol. Oncol.