FIGURE

Fig. S1

ID

ZDB-FIG-150416-29

Publication

Wen et al., 2015 - Sox4 regulates choroid fissure closure by limiting hedgehog signaling during ocular morphogenesis

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Fig. S1

Co-localization of sox4a/b with cell specific markers at 72 hpf. (A-B′′) FISH for sox4a and sox4b followed by immunostaining for the ganglion and amacrine cell marker HuC/D. Whereas sox4a did not co-localize with HuC/D (A-A′′), some sox4b-positive cells co-localized with HuC/D (B-B′′, arrows). (C-D′′) FISH for sox4a and sox4b followed by immunostaining for the horizontal cell marker Prox1. Sox4a did not co-localize with Prox1 (C-C′′); however some sox4b-positive cells in the outer half of the INL co-localized with Prox1 (D-D′′, arrows). (E-F′′) FISH for sox4a and sox4b followed by immunostaining for the bipolar cell marker PKC-α. No co-localization with PKC-α was detected. (G-H′′) FISH for sox4a and sox4b was performed in embryos transgenic for gfap:GFP which labels Müller glial cells. No co-localization with Müller cells was observed. All scale bars equal 25 µm. GCL, ganglion cell layer; INL, inner nuclear layer.

Expression Data

Genes:	GFP sox4a sox4b
Fish:	WT mi2001Tg
Anatomical Terms:	amacrine cell horizontal cell Muller cell retinal ganglion cell retinal ganglion cell layer retinal inner nuclear layer
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 399(1), Wen, W., Pillai-Kastoori, L., Wilson, S.G., Morris, A.C., Sox4 regulates choroid fissure closure by limiting hedgehog signaling during ocular morphogenesis, 139-53, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.