FIGURE

Fig. 3

ID

ZDB-FIG-150325-45

Publication

Staudt et al., 2015 - A panel of recombinant monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining

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Fig. 3

Antibodies detect neural receptor proteins within discrete subcellular localisations. (A) Anti-Sema4c staining in the anterior neuroepithelium. (B) Anti-Kita labels the anterior (ac) and postoptic (poc) commissures and is also enriched at the periphery of cells (arrow). (C) Anti-Kita also stained axons of the ventral nerve cord (vnc) and the surface of hindbrain cells. Anti-CD276 staining in the developing midbrain (D), and tectum (E). (F) Anti-Igsf21b stained axon-rich regions in the anterior forebrain where it was often concentrated in discrete puncta (arrow). Views are anterior to the left and dorsal for A and E, lateral for B, C and F, ventral for D. Scale bars represent 100 µm in (A) and 50 µm in (B–F).

Expression Data

Genes:	cd276 igsf21b kita sema4c
Antibodies:	Ab1-cd276 Ab1-igsf21b Ab1-sema4c Ab2-kita
Fish:	WT
Anatomical Terms:	anterior commissure caudal commissure forebrain anterior region hypothalamus neuron midbrain olfactory bulb neuroepithelial cell olfactory epithelium telencephalon neuron ventral spinal nerve axon
Stage Range:	Pec-fin to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.