FIGURE

Fig. 4

ID

ZDB-FIG-150325-46

Publication

Staudt et al., 2015 - A panel of recombinant monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining

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Fig. 4

The addition of different tags facilitates multiplex staining. (A) Schematics showing the addition of C-terminal Bio-6-His and FLAG tags to plasmids. The region encoding the specific antibodies are flanked by unique NotI and AscI sites facilitating antibody tag switching by subcloning. (B–D) The same 5 dpf embryo was simultaneously stained with both the biotin-tagged anti-Lrrc3b and FLAG-tagged anti-Sema4c antibodies. The staining of anti-Lrrc3b in green (B) and anti-Sema4c in red (C), together with the merged images (D) at the same site are shown. Staining patterns are described in the main text. Scale bars represent 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.