Fig. 2

Immunohistochemistry of the embryonic zebrafish brain with monoclonal antibodies to neural receptors and secreted proteins. The embryo stage and transcript/protein are indicated within each subpanel and staining patterns described within the main text. Transcript localisation is shown by wholemount in situ hybridisation as brightfield images; images of antibody staining are taken by confocal microscopy (red); nuclei are stained with DAPI in G^II (green). Note that some antibody staining, for example Igsf21a in the ventral diencephalon (B^I), is outside of the optical plane. Orientation of the embryos is always anterior to the left with ventral views in A, A^I, D^I, I^I and J^I; lateral in A^II, C, C^I, D, D^II, D^III, E, E^I, G, G^I, H, H^I, J and J^III; and dorsal in A^III,B, B^I, F, F^I, G^II, G^III, I and J^II. Scale bars represent 100 µm in C^I and E^I, for all other images 50 µm. Abbreviations used in all figures are: ac = anterior commissure, dvdt = dorso–ventral diencephalic tract, epi = epithalamus, hind = hindbrain, hypo = hypothalamus, MHB = mid-hindbrain boundary, mid = midbrain, ob: olfactory bulb, oe = olfactory epithelium, poc = postoptic commissure, sot = supraoptic tract, tec = tectum, teg = tegmentum, tel = telencephalon, th = thalamus, tpc = tract of the posterior commissure, tpoc = tract of the postoptic commissure, vlt = ventral longitudinal tract, vnc =  ventral nerve cord, hpf = hours post-fertilisation, dpf = days post-fertilisation.