FIGURE

Fig. 8

ID

ZDB-FIG-150113-17

Publication

Muto et al., 2014 - Nipbl and Mediator Cooperatively Regulate Gene Expression to Control Limb Development

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Fig. 8

Functional interactions between Nipbl and Med12 in pectoral fin development.

(A) hox gene expression in pectoral fin buds of Med12-deficient embryos examined by ISH at 36 hpf. Dorsal views with anterior to the top. (B) Lateral views of pectoral fins in living larvae at 76 hpf in controls or injected with 0.5 ng med12-MO alone (low-med12-MO). (C) Pectoral fin lengths in larvae injected with low-med12-MO alone or combined with low amounts of nipbl-MOs (0.05 ng nipbla-MO+0.75 ng of nipblb-MO; low-nipbla/b-MO). Medians: 410.1 μm, n = 16 (control), 382.2 μm, n = 24 (low-nipbla/b-MOs), 385.4 μm, n = 16 (low-med12-MO alone), and 341.4 μm, n = 16 (low-med12-MO+low-nipbla/b-MOs). Asterisks indicate statistical significance (p-values<0.001). (D) hox expression in larvae injected with low-med12-MO alone or combined with low nipbla/b-MO. Dorsal views, anterior to the top.

Expression Data

Genes:	hoxa10b hoxa13b hoxc6a hoxc8a hoxd3a hoxd4a hoxd11a
Fish:	AB AB + MO5-med12 AB + MO1-nipbla + MO1-nipblb + MO5-med12
Knockdown Reagents:	MO1-nipbla MO1-nipblb MO5-med12
Anatomical Term:	pectoral fin bud
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO5-med12 AB + MO1-nipbla + MO1-nipblb + MO5-med12
Knockdown Reagents:	MO1-nipbla MO1-nipblb MO5-med12
Observed In:	embryonic pectoral fin morphogenesis pectoral fin pectoral fin bud embryonic pectoral fin morphogenesis
Stage Range:	Prim-25 to Protruding-mouth

Phenotype Detail

Acknowledgments

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