FIGURE

Fig. 3

ID

ZDB-FIG-150113-13

Publication

Muto et al., 2014 - Nipbl and Mediator Cooperatively Regulate Gene Expression to Control Limb Development

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Fig. 3

Reduction of genes involved in the shh-related gene regulatory cassette in developing pectoral fin mesenchyme of Nipbl-deficient embryos.

Expression of fin mesenchymal genes at indicated stages in control and Nipbl-deficient embryos examined by ISH. Dorsal views, anterior to the top. (A, B) Expression of shha and ptch2 in pectoral fin buds (arrows) was significantly reduced in Nipbl-deficient embryos, while midline, neural tube expression was unaffected. Expression in endoderm derived-tissues (asterisks) is also reduced. (C) Expression of hand2 was also significantly reduced in stage-matched pectoral fin buds. hand2 expression in the fin buds and posterior lateral plate mesoderm is marked by brackets and arrowheads, respectively. (D) gli3 expression was not significantly affected in a stage-matched comparison.

Expression Data

Genes:	gli3 hand2 ptch2 shha
Fish:	AB AB + MO1-nipbla + MO1-nipblb
Knockdown Reagents:	MO1-nipbla MO1-nipblb
Anatomical Terms:	pectoral fin bud posterior lateral plate mesoderm spinal cord
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-nipbla + MO1-nipblb
Knockdown Reagents:	MO1-nipbla MO1-nipblb
Observed In:	pectoral fin bud pectoral fin bud regulation of smoothened signaling pathway
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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