FIGURE

Fig. 2

ID

ZDB-FIG-140919-2

Publication

Pi-Roig et al., 2014 - Distinct tissue-specific requirements for the zebrafish tbx5 genes during heart, retina and pectoral fin development

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Fig. 2

tbx5 morphants exhibit cardiac jogging and lefty2 expression defects. (a-a′′) tbx5a morphant phenotypes. (b-b′′) tbx5b morphants. (c-e′′) Left, right and middle jog phenotypes obtained by co-injection of tbx5a and tbx5b MOs at 0.5 ng (c-c′′), 1.5 ng (d-d′′) or 3 ng (e-e′′) of each MO. (f) Control (ctrl) morphant. (g) Quantification of the phenotypes. (h-j) lefty2 expression in control (h) and double-morphant (i,j) 22-somite stage embryos. (k) Quantification of the phenotypes. A χ² test has been used to assess significant differences between groups (**p < 0.001). All images are dorsal views with anterior to the top, and myl7 expression is used to highlight the developing heart tube in a-f.

Expression Data

Genes:	lft2 myl7
Fish:	WT WT + MO1-tbx5a WT + MO1-tbx5b WT + MO1-tbx5a + MO1-tbx5b
Knockdown Reagents:	MO1-tbx5a MO1-tbx5b
Anatomical Terms:	heart rudiment heart tube
Stage Range:	20-25 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-tbx5a WT + MO1-tbx5b WT + MO1-tbx5a + MO1-tbx5b
Knockdown Reagents:	MO1-tbx5a MO1-tbx5b
Observed In:	heart jogging
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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