FIGURE

Fig. 1

ID

ZDB-FIG-140919-1

Publication

Pi-Roig et al., 2014 - Distinct tissue-specific requirements for the zebrafish tbx5 genes during heart, retina and pectoral fin development

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Fig. 1

Knock-down of tbx5 genes causes cardiac looping defects. (a-c) Embryos injected with control MO or sub-optimal concentrations of tbx5a or tbx5b MOs. (d-d′′) tbx5a morphant phenotypes. (e-e′) tbx5b-morphant phenotypes. (f-h′′) Double knock-down of tbx5 genes (0.5 ng each MO (f-f′′), 1.5 ng each MO (g-g′′) and 3 ng each MO (h-h′′)). (i) Quantification of the degree of looping phenotypes: wt, complete; phenotype, incomplete looping s.o., sub-otimal. (j) Quantification of the looping orientation phenotypes. A χ² statistic has been calculated to assess significant differences between groups (**p < 0.001, *p < 0.05). Images are frontal views of 48 hpf embryos, and myl7 expression is used to highlight the developing heart.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-tbx5a WT + MO1-tbx5b WT + MO1-tbx5a + MO1-tbx5b
Knockdown Reagents:	MO1-tbx5a MO1-tbx5b
Observed In:	heart heart looping
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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