FIGURE

Fig. 9

ID

ZDB-FIG-140915-19

Publication

Skobo et al., 2014 - Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development

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Fig. 9

Knockdown of ambra1 interferes with myosin organization in slow and fast muscles and with myosepta.

Lateral views of 3 dpf embryos labeled with the F59 antibody (slow muscle fibers) and with the F310 antibody (fast muscle fibers), showing abnormally organized myofilaments in ATG-morphant embryos when compared to WT and 5 m-control embryos. Slow fibers are thinner in ATG-morphant embryos, whereas fast fibers of splice-morphant embryos display a wavy phenotype, visibly different from controls. The asterisks indicate broken or missing muscle fibers. Laminin labeling highlights the loss of the V-shape arrangement of somites and reveals interrupted myosepta (arrowheads) in ATG-morphant embryos. Defects in myosin organization and myosepta structure are rescued with the by co-injection with human AMBRA1 mRNA. Anterior is to the left and dorsal up.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-ambra1a AB + MO1-ambra1b AB + MO2-ambra1a AB + MO2-ambra1b AB + MO1-ambra1a + MO1-ambra1b
Knockdown Reagents:	MO1-ambra1a MO1-ambra1b MO2-ambra1a MO2-ambra1b
Observed In:	fast muscle cell muscle cell myoseptum myosin filament organization skeletal myofibril assembly somite
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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