FIGURE

Fig. 8

ID

ZDB-FIG-140915-18

Publication

Skobo et al., 2014 - Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development

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Fig. 8

Ultrastructural analysis of ambra1 morphants muscles reveals disorganized subcellular structures.

When compared to WT and 5-control (not shown) embryos, ambra1 morphant embryos display alterations of mitochondria (row A), nuclei (row B) and triads (row C), perturbation of the hexagonal arrangement of thick and thin filaments (row D), and dilations of the endoplasmic reticulum (row E). Muscles of morphant embryos show the presence of areas with reduced thin filaments (black circles in row D) adjacent to more normal-appearing hexagonal structures (white circles in row D). Co-injected double morphant embryos show exacerbated defects of these structures, whereas defects are barely evident in splice-morphants.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-ambra1a AB + MO1-ambra1b AB + MO1-ambra1a + MO1-ambra1b
Knockdown Reagents:	MO1-ambra1a MO1-ambra1b
Observed In:	muscle mitochondrion muscle myosin filament muscle sarcoplasmic reticulum muscle striated muscle thin filament muscle cell muscle cell nucleus
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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