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Fig. 2

ID

ZDB-FIG-140709-35

Publication

Feng et al., 2010 - Dhrs3a regulates retinoic acid biosynthesis through a feedback inhibition mechanism

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Fig. 2

Strategy for microarray identification of RA-regulated genes. Wild-type embryos were treated with RA, RA signaling antagonist (AGN193109) or DMSO (control) as shown. Treated embryos were collected at the 2–4 somite stage (about 11 hpf). A subset of treated embryos were fixed for in situ hybridization with mafBa, erm, or hoxd4, to confirm the drug treatment effect, and the remainder was used for RNA harvest. Four separate treatments were performed. After a single cycle of cRNA synthesis, the hybridization was carried out on Affymetrix Zebrafish Genome Expression chips. Selected hits were validated by in situ hybridization.

Expression Data

Genes:	etv5b hoxd4a mafba
Fish:	AB
Conditions:	chemical treatment: pharmaceutical chemical treatment: all-trans-retinoic acid
Anatomical Terms:	presumptive rhombomere 4 presumptive rhombomere 5 presumptive rhombomere 6 presumptive rhombomere 7 presumptive rhombomere 8 whole organism anterior region whole organism posterior region
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 338(1), Feng, L., Hernandez, R.E., Waxman, J.S., Yelon, D., and Moens, C.B., Dhrs3a regulates retinoic acid biosynthesis through a feedback inhibition mechanism, 1-14, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.