FIGURE

Fig. 8

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ZDB-FIG-140709-41

Publication

Feng et al., 2010 - Dhrs3a regulates retinoic acid biosynthesis through a feedback inhibition mechanism

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Fig. 8

Dhrs3a knockdown effects nervous system patterning. (A–C) in situ hybridization of hoxd4a (blue), pax2a (red in mid-hindbrain boundary, otic vesicles and somites) and egr2b (red in rhombomere 3 and 5) in wt (A), dhrs3a MO1 (B) and MO3 (C)-injected embryos. Dotted arrows indicate the distance from the r6/7 boundary, marked by the anterior limit of hoxd4a expression, to the second somite (indicated by the anterior limit of pax2a expression). Solid arrows indicate the distance from the r3/4 boundary to the second somite. Both distances are longer in dhrs3a knockdown embryos. (D–G) Knockdown of Dhrs3 enhances the posteriorized hindbrain phenotype of cyp26a1 mutant embryos: (D) wt; (E) cyp26a1-/gir mutant; (F, G) cyp26a1-/gir mutant injected with dhrs3a MO3 (F) or MO1 (G).

Expression Data

Genes:	egr2b hoxd4a pax2a
Fish:	AB WT cyp26a1^rw716/rw716 AB + MO1-dhrs3a + MO4-tp53 AB + MO2-dhrs3a + MO4-tp53 WT + MO1-dhrs3a + MO4-tp53 WT + MO2-dhrs3a + MO4-tp53 cyp26a1^rw716/rw716 + MO1-dhrs3a + MO4-tp53 cyp26a1^rw716/rw716 + MO2-dhrs3a + MO4-tp53
Knockdown Reagents:	MO1-dhrs3a MO2-dhrs3a MO4-tp53
Anatomical Terms:	hindbrain neural plate midbrain hindbrain boundary neural plate otic placode presumptive rhombomere 3 presumptive rhombomere 5 somite
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 338(1), Feng, L., Hernandez, R.E., Waxman, J.S., Yelon, D., and Moens, C.B., Dhrs3a regulates retinoic acid biosynthesis through a feedback inhibition mechanism, 1-14, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.