FIGURE

Fig. 4

ID

ZDB-FIG-140522-53

Publication

Ouchi et al., 2014 - The heterochronic genes lin-28a and lin-28b play an essential and evolutionarily conserved role in early zebrafish development

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Fig. 4

Effect of lin-28a and lin-28b knockdown on cell proliferation.

A. Confocal images of BrdU (Red) incorporation in lin-28a and lin-28b morphant embryos at 5hpf. Both lin-28a and lin-28b MO-injected embryos were exposed to BrdU at 4 hpf for 1 hour. The nuclei were stained with DAPI (blue). B. Percentage of BrdU incorporated cells in lin-28a and lin-28b morphants embryos. The percentage of BrdU-incorporated cells was calculated by dividing the number of BrdU-positive cells by the total number of cells as determined by DAPI-stained cells. The percentage of BrdU-positive cells was significantly lower in both lin-28a MO- and lin-28b MO-injected embryos compared with control MO-injected embryos (***p<0.001, control MO vs. lin-28a MO, control MO vs. lin-28b MO, two-tailed test, n = 10, mean ± SEM).

Expression Data

Genes:	dre-let-7b trim71
Fish:	AB AB + MO1-lin28b AB + MO3-lin28ab
Knockdown Reagents:	MO1-lin28b MO3-lin28ab
Anatomical Terms:	central nervous system whole organism
Stage Range:	30%-epiboly to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-lin28b AB + MO3-lin28ab
Knockdown Reagents:	MO1-lin28b MO3-lin28ab
Observed In:	cell population proliferation
Stage:	30%-epiboly

Phenotype Detail

Acknowledgments

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