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Fig. 4

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ZDB-IMAGE-140522-48
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Figures for Ouchi et al., 2014
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Fig. 4 Effect of lin-28a and lin-28b knockdown on cell proliferation.

A. Confocal images of BrdU (Red) incorporation in lin-28a and lin-28b morphant embryos at 5hpf. Both lin-28a and lin-28b MO-injected embryos were exposed to BrdU at 4 hpf for 1 hour. The nuclei were stained with DAPI (blue). B. Percentage of BrdU incorporated cells in lin-28a and lin-28b morphants embryos. The percentage of BrdU-incorporated cells was calculated by dividing the number of BrdU-positive cells by the total number of cells as determined by DAPI-stained cells. The percentage of BrdU-positive cells was significantly lower in both lin-28a MO- and lin-28b MO-injected embryos compared with control MO-injected embryos (***p<0.001, control MO vs. lin-28a MO, control MO vs. lin-28b MO, two-tailed test, n = 10, mean ± SEM).

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