FIGURE

Fig. 3

ID

ZDB-FIG-131211-59

Publication

Arrington et al., 2013 - Sdc2 and Tbx16 regulate Fgf2-dependent epithelial cell morphogenesis in the ciliated organ of asymmetry

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Fig. 3

Cell-autonomous regulation of KV ciliogenesis and asymmetric fluid flow by Sdc2. (A-D) KV cilia labeled with anti-tubulin antibody were shorter in global sdc2 (B) and DFC^sdc2MO (C) morphant zebrafish embryos than in wild-type (A) and yolk^sdc2MO (D) embryos. (E,F) Cilia length and number were reduced in global and DFC^sdc2MO morphants (global morphants: seven embryos, 267 cilia; DFC^sdc2MO: ten embryos, 177 cilia) compared with wild-type and yolk^sdc2MO embryos (wild type: six embryos, 373 cilia; yolk^sdc2MO: six embryos, 287 cilia), ^*P<0.05 (Student’s t-test). Error bars indicate s.e.m. (G,H) KV fluid flow was altered in sdc2 morphants. Bead tracks are circular in wild-type embryos (G) but disorganized in sdc2 morphants (H).

Expression Data

Antibody:	Ab1-tuba
Fish:	AB AB + MO5-sdc2
Knockdown Reagent:	MO5-sdc2
Anatomical Term:	Kupffer's vesicle motile cilium
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO5-sdc2
Knockdown Reagent:	MO5-sdc2
Observed In:	epithelial cilium movement involved in determination of left/right asymmetry Kupffer's vesicle motile cilium
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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