FIGURE

Fig. 6

ID

ZDB-FIG-131211-62

Publication

Arrington et al., 2013 - Sdc2 and Tbx16 regulate Fgf2-dependent epithelial cell morphogenesis in the ciliated organ of asymmetry

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Fig. 6

fgf2 mRNA rescues KV morphogenesis and cilia number, but not cilia length, in sdc2 morphants. (A-C) Co-injection of sdc2 AUG MO and fgf2 mRNA rescued KV morphology (labeled with anti-ζPKC antibody). (D-H) Among rescued KV, cilia number in sdc2 morphants was partially rescued by fgf2 mRNA co-injection, but cilia length was not rescued (wild type: seven embryos, 444 cilia; sdc2 morphants + fgf2 mRNA: eight embryos, 416 cilia; compare with sdc2 morphants alone, Fig. 3E,F). ^*P<0.001 (Student’s t-test). Error bars indicate s.e.m. (I) Proposed genetic pathway by which Sdc2 controls KV morphogenesis and ciliogenesis. DFC-expressed Sdc2 is required for tbx16 expression, which in turn regulates fgf2-dependent KV morphogenesis and fgf2-independent ciliogenesis.

Expression Data

Antibodies:	Ab1-prkcz Ab1-tuba
Fish:	AB + MO5-sdc2
Condition:	chemical treatment: messenger RNA
Knockdown Reagent:	MO5-sdc2
Anatomical Terms:	Kupffer's vesicle apical plasma membrane Kupffer's vesicle motile cilium
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO5-sdc2
Condition:	chemical treatment: messenger RNA
Knockdown Reagent:	MO5-sdc2
Observed In:	Kupffer's vesicle Kupffer's vesicle motile cilium Kupffer's vesicle development
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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