Fig. 3

Fig. 3 Cell-autonomous regulation of KV ciliogenesis and asymmetric fluid flow by Sdc2. (A-D) KV cilia labeled with anti-tubulin antibody were shorter in global sdc2 (B) and DFC^sdc2MO (C) morphant zebrafish embryos than in wild-type (A) and yolk^sdc2MO (D) embryos. (E,F) Cilia length and number were reduced in global and DFC^sdc2MO morphants (global morphants: seven embryos, 267 cilia; DFC^sdc2MO: ten embryos, 177 cilia) compared with wild-type and yolk^sdc2MO embryos (wild type: six embryos, 373 cilia; yolk^sdc2MO: six embryos, 287 cilia), ^*P<0.05 (Student’s t-test). Error bars indicate s.e.m. (G,H) KV fluid flow was altered in sdc2 morphants. Bead tracks are circular in wild-type embryos (G) but disorganized in sdc2 morphants (H).