FIGURE

Fig. 5

ID

ZDB-FIG-131031-18

Publication

Jao et al., 2013 - Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

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Fig. 5

Biallelic disruption of ddx19 by Cas9 generates null-like phenotypes. ddx19 gRNA (150 pg) and nls-zCas9-nls RNA (150 pg) were injected into wild-type embryos. (A–G) Lateral views of the wild-type (A and E), ddx19-targeted (B, C, and F), and homozygous ddx19 mutants (ddx19^{hi1464/hi1464} ) (D and G) embryos. ddx19-targeted embryos recapitulated several ddx19 null phenotypes (e.g., brain necrosis, small eyes, and curved body axis), albeit to a lesser extent. (H) T7EI assays showed high mutagenesis rates at the ddx19 target (87–91%) in six randomly selected ddx19-targeted embryos. (Scale bars: in D, 300 μm; in G, 500 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ddx19b^{hi1464Tg/hi1464Tg} ddx19b^zf452/zf452
Observed In:	brain eye whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA