FIGURE

Fig. 3

ID

ZDB-FIG-131031-16

Publication

Jao et al., 2013 - Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

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Fig. 3

Biallelic disruption of tyrosinase (tyr) by Cas9 generates mosaic pigmentation phenotypes. tyr gRNA (30 pg) and nls-zCas9-nls RNA (150 pg) were injected into wild-type embryos. (A–E) Lateral views of wild-type (A) and tyr-targeted embryos (B–E) at 2 dpf. tyr-targeted embryos showed different degrees of hypopigmentation, some of them were almost unpigmented (e.g., E). (F–K) Dorsal view of the wild-type (F and I) and tyr-targeted larvae (G, H, J, and K) at 7 dpf (F–H) and 14 dpf (I–K). Mosaic pigmentation defects in tyr-targeted larvae persisted to later stages. (L) T7EI assays showed high mutagenesis rates at the tyr target (93–97%) in six randomly selected tyr-targeted embryos. (Scale bars: 500 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tyr^zf451/zf451
Observed In:	melanin biosynthetic process from tyrosine
Stage Range:	Long-pec to Days 14-20

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA