FIGURE

Fig. 4

ID

ZDB-FIG-131031-17

Publication

Jao et al., 2013 - Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

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Fig. 4

Biallelic disruption of golden (gol) by Cas9 generates mosaic pigmentation phenotypes. gol gRNA (50 pg) and nls-zCas9-nls RNA (150 pg) were injected into wild-type embryos. (A–F) Lateral views of wild-type (A and E) and gol-targeted embryos (B–D and F) at 2 dpf. Whereas the wild-type embryo showed darkly pigmented retinal pigment epithelium (RPE), gol-targeted embryos had patches of unpigmented cells in RPE (B–D). The skin melanophores in gol-targeted embryos were also hypopigmented (F). (G) T7EI assays showed high mutagenesis rates at the gol target (80–98%) in six randomly selected gol-targeted embryos. (H–K) Lateral views of wild-type (H and J) and gol-targeted (I and K) juvenile fish at 54 dpf. gol-targeted fish had patches of pale melanophores (dashed boxes) among the dark skin stripes. (Scale bars: in D, 300 μm; in F, 500 μm; in K, 2 mm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc24a5^zf450/zf450
Observed In:	integument melanocyte retinal pigmented epithelium pigment granule
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA