FIGURE

Fig. 4

ID

ZDB-FIG-130801-20

Publication

Nichols et al., 2013 - barx1 represses joints and promotes cartilage in the craniofacial skeleton

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Fig. 4

Ectopic joint cells are present in barx1 mutant zebrafish. (A,B) All cells were stained with SYTO59 (red), and chondrocytes transgenically labeled with EGFP (sox9a:EGFP, green) in 4 dpf animals. (C,D) 66 hpf larvae were subjected to in situ hybridization to label cartilage (sox9a, green) and joint cells (chd, red). (E,F) Live larvae with chondrocyte cell membranes transgenically labeled with RFP (sox10:mRFP, red) and joint region cells labeled with EGFP (trps1:EGFP, green) were imaged. (G,H) 72 hpf larvae with chondrocytes transgenically labeled with EGFP (sox9a:EGFP, green) were stained with anti-collagen type II antibody (red). Skeletal elements are oriented and labeled as in Fig. 1F. Arrowhead indicates boundary between distinct chondrocyte populations. All images are single confocal sections. Scale bars: 50 μm.

Expression Data

Genes:	chrd EGFP mRFP
Fish:	WT barx1^fh331/fh331 sox9a^zc81Tg trps1^j1271aGt; vu234Tg barx1^fh331/fh331; sox9a^zc81Tg barx1^fh331/fh331; trps1^j1271aGt; vu234Tg
Anatomical Terms:	chondrocyte chondrocyte cell outer membrane joint Meckel's cartilage palatoquadrate arch
Stage Range:	Pec-fin to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	barx1^fh331/fh331; sox9a^zc81Tg
Observed In:	chondrocyte joint
Stage:	Day 4

Phenotype Detail

Acknowledgments

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