Fig. S1
Skeletal phenotype variability in barx1 mutant zebrafish. (A-D) Zebrafish heterozygous for barx1fh330 and barx1fh331 alleles were crossed and offspring were stained with Alcian Blue and Alizarin Red, dissected and imaged with transmtted light. Genotyped trans-heterozygotes displayed barx1-associated phenotypes revealing that these two alleles fail to complement. Anterior is towards the left; left is upwards. (E-P) Alcian Blue- and Alizarin Red-stained larvae were dissected and flat mounts imaged to reveal the range of phenotypic severity found in barx1 mutants. Anterior is towards the left; dorsal is upwards. Scale bar: 10 μm. |