FIGURE

Fig. S8

ID

ZDB-FIG-130411-1

Publication

Boglev et al., 2013 - Autophagy induction is a tor- and tp53-independent cell survival response in a zebrafish model of disrupted ribosome biogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S8

Autophagic flux in tti^s450 larvae is not abrogated by Tor pathway activation. (A–D) Enhancing Torc1 activity by ablating Tsc2 activity in tti^s450 larvae does not change their gross morphology at 120 hpf. Compound mutants (tti^s450;Tsc2^vu242/vu242) (D) are essentially indistinguishable from tti^s450 larvae (C). Other panels show WT (A) and Tsc2^vu242/vu242 mutant (B) larvae. (E,F) Western blot analysis of p-RPS6 and LC3 demonstrates that tti^s450;Tsc2^vu242/vu242 compound mutants at 96 hpf contain higher levels of p-RPS6 than tti^s450 mutants due to increased Tor activity, yet LC3II levels are comparable between the two genotypes (refer to right hand half of the Western blot, where the larvae were pre-treated with chloroquine). p-RPS6 and LC3II levels are not significantly different between WT and tsc2^vu242/vu242 larvae in the presence of chloroquine. Actin was used as a loading control. The levels of LC3II were quantitated by densitometric analysis of three independent Western blots. Data are represented as mean +/ SD, *p<0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.