FIGURE

Fig. S4

ID

ZDB-FIG-130411-5

Publication

Boglev et al., 2013 - Autophagy induction is a tor- and tp53-independent cell survival response in a zebrafish model of disrupted ribosome biogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

LC3II-containing autophagosomes are found in multiple tissues in tti^s450 larvae at 72 hpf and 120 hpf. (A–H) RNA encoding a mCherry-LC3 fusion protein was injected into the yolk of 1–4 cell zebrafish embryos derived from a pairwise mating of tti^s450/+ heterozygotes (on the gutGFP background) and allowed to develop until the indicated time-point in the presence of chloroquine for the final 14 h. Maximum intensity projection images of a z series of confocal sections through WT [A, A′ (boxed area in A), C, E, E′ (boxed area in E) and G] and tti^s450 larvae [B, B′ (boxed area in B), D, F, F′ (boxed area in F) and H] showing accumulated autophagosomes (red puncta) in the brain, eye and digestive organs (marked by GFP fluorescence in C, D) at 72 hpf (A–D) and 120 hpf (E–H). Scale bars = 50 μM. b, brain; e, eye; ib, intestinal bulb; f, fin; y, yolk; p, pancreas.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.