ZFIN Image: Boglev et al., 2013, Fig. S8

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Figure Caption

Fig. S8 Autophagic flux in tti^s450 larvae is not abrogated by Tor pathway activation. (A–D) Enhancing Torc1 activity by ablating Tsc2 activity in tti^s450 larvae does not change their gross morphology at 120 hpf. Compound mutants (tti^s450;Tsc2^vu242/vu242) (D) are essentially indistinguishable from tti^s450 larvae (C). Other panels show WT (A) and Tsc2^vu242/vu242 mutant (B) larvae. (E,F) Western blot analysis of p-RPS6 and LC3 demonstrates that tti^s450;Tsc2^vu242/vu242 compound mutants at 96 hpf contain higher levels of p-RPS6 than tti^s450 mutants due to increased Tor activity, yet LC3II levels are comparable between the two genotypes (refer to right hand half of the Western blot, where the larvae were pre-treated with chloroquine). p-RPS6 and LC3II levels are not significantly different between WT and tsc2^vu242/vu242 larvae in the presence of chloroquine. Actin was used as a loading control. The levels of LC3II were quantitated by densitometric analysis of three independent Western blots. Data are represented as mean +/ SD, *p<0.05.

Acknowledgments

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