FIGURE

Fig. 3

ID

ZDB-FIG-120209-19

Publication

Zhang et al., 2012 - Zebrafish Models for Dyskeratosis Congenita Reveal Critical Roles of p53 Activation Contributing to Hematopoietic Defects through RNA Processing

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Fig. 3

Activation of p53 pathway and rescue of hematopoietic defects in zebrafish dkc1 and nola1 deficiency.

(A) and (C) Expression of genes of p53 pathway was up-regulated in both dkc1 morphant (A) and nola1 mutant (C). Expression level of wild type embryos of related genes was normalized as 1.0. (B) Knockdown of p53 by injection of p53 MO rescued the reduction of HSC in dkc1 morphants (compare c with e, d with f). C-myb expression at 3 dpf (a, c and e); runx1 expression at 30 hpf (b, d and f). (D) Red blood cells were restored in nola1 and p53 double mutatant embryos (red arrow in a–d). 25 embryos at 4 dpf were examined for each group. And the results shown here were representative for three independent experiments.

Expression Data

Genes:	baxa ccng1 myb runx1 tp53
Fish:	WT WT + MO1-dkc1 WT + MO1-dkc1 + MO4-tp53 gar1^{zf303Tg/zf303Tg}
Knockdown Reagents:	MO1-dkc1 MO4-tp53
Anatomical Terms:	hematopoietic stem cell whole organism
Stage Range:	Prim-15 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tp53^zdf1/zdf1 WT + MO1-dkc1 WT + MO1-dkc1 + MO4-tp53 gar1^{zf303Tg/zf303Tg} tp53^zdf1/zdf1; gar1^{zf303Tg/zf303Tg}
Knockdown Reagents:	MO1-dkc1 MO4-tp53
Observed In:	hematopoietic stem cell nucleate erythrocyte signal transduction by p53 class mediator
Stage Range:	Prim-15 to Day 4

Phenotype Detail

Acknowledgments

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