FIGURE

Fig. 3

ID

ZDB-FIG-111115-13

Publication

O'Brien et al., 2011 - Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish

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Fig. 3

Analysis of podocyte, neck and interrenal development by in situ hybridization following morpholino knockdown of wt1a, foxc1a and/or rbpj. Each transcription factor was knocked down either by itself or in combination with each of the other factors as indicated. The expression analysis was carried out at 15 somites, 24 hpf, 36 hpf and 48 hpf. wt1b⁺ = podocytes; cdh17⁺ = non-podocyte renal epithelia; slc20a1a⁺ = first proximal tubule segment; nephrin⁺ = mature podocyte; podocalyxin⁺ = mature podocyte; nr5a1a⁺ = interrenal cells; pax2a⁺ = podocytes and neck segment (15 somite), neck segment (48 hpf). Analysis of hey1 expression by in situ hybridization was performed at 15 somites under single and double knockdown conditions. Arrows point to podocyte expression of hey1.

Expression Data

Genes:	cdh17 hey1 nphs1 nr5a1a pax2a podxl slc20a1a wt1b
Fish:	TU TU + MO1-foxc1a TU + MO3-wt1a TU + MO1-foxc1a + MO3-wt1a TU + MO4-rbpja,rbpjb TU + MO1-foxc1a + MO4-rbpja,rbpjb TU + MO3-wt1a + MO4-rbpja,rbpjb
Knockdown Reagents:	MO1-foxc1a MO3-wt1a MO4-rbpja,rbpjb
Anatomical Terms:	interrenal primordium mesoderm pronephric duct pronephric glomerulus pronephric podocyte pronephric proximal straight tubule pronephric tubule somite
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-foxc1a TU + MO3-wt1a TU + MO1-foxc1a + MO3-wt1a TU + MO4-rbpja,rbpjb TU + MO1-foxc1a + MO4-rbpja,rbpjb TU + MO3-wt1a + MO4-rbpja,rbpjb
Knockdown Reagents:	MO1-foxc1a MO3-wt1a MO4-rbpja,rbpjb
Observed In:	apoptotic process interrenal primordium pronephric duct pronephric podocyte pronephric proximal convoluted tubule pronephric tubule
Stage Range:	14-19 somites to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 358(2), O'Brien, L.L., Grimaldi, M., Kostun, Z., Wingert, R.A., Selleck, R., and Davidson, A.J., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish, 318-30, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.