ZFIN Image: O'Brien et al., 2011, Fig. 3

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Fig. 3

Analysis of podocyte, neck and interrenal development by in situ hybridization following morpholino knockdown of wt1a, foxc1a and/or rbpj. Each transcription factor was knocked down either by itself or in combination with each of the other factors as indicated. The expression analysis was carried out at 15 somites, 24 hpf, 36 hpf and 48 hpf. wt1b⁺ = podocytes; cdh17⁺ = non-podocyte renal epithelia; slc20a1a⁺ = first proximal tubule segment; nephrin⁺ = mature podocyte; podocalyxin⁺ = mature podocyte; nr5a1a⁺ = interrenal cells; pax2a⁺ = podocytes and neck segment (15 somite), neck segment (48 hpf). Analysis of hey1 expression by in situ hybridization was performed at 15 somites under single and double knockdown conditions. Arrows point to podocyte expression of hey1.

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Acknowledgments

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Reprinted from Developmental Biology, 358(2), O'Brien, L.L., Grimaldi, M., Kostun, Z., Wingert, R.A., Selleck, R., and Davidson, A.J., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish, 318-30, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.