FIGURE

Fig. 5

ID

ZDB-FIG-111018-22

Publication

Mouillesseaux et al., 2011 - Mutation in utp15 Disrupts Vascular Patterning in a p53-Dependent Manner in Zebrafish Embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Analysis of endogenous utp15 expression by whole-mount in situ hybridization.

A, utp15 is expressed ubiquitously in developing embryos at early stages. B, Expression was restricted to neural and vascular tissues after one day of development. B′, 2x magnification of the rectangular region indicated in (B), showing expression in the dorsal aorta and cardinal vein. Red bar, approximate location of the vibratome cross-section shown in (B′′). C–D, utp15 was dramatically reduced in mutant embryos, as shown by in situ hybridization (C) and qRT-PCR (D) analyses. A–C, Embryos shown are representative of triplicate experiments performed on a minimum of 5 embryos per condition. D, Data shown are the average fold changes in expression±SEM from two replicate qRT-PCR assays using pooled RNA from 20 embryos per condition.

Expression Data

Gene:	utp15
Fish:	la116Tg utp15^{la1908/la1908}; la116Tg
Anatomical Terms:	brain dorsal aorta posterior cardinal vein vasculature whole organism
Stage Range:	14-19 somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One