FIGURE

Fig. 10

ID

ZDB-FIG-110909-78

Publication

Rothschild et al., 2011 - CaMK-II is a PKD2 target that promotes pronephric kidney development and stabilizes cilia

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Fig. 10

CaMK-II and PKD2 act in the same molecular pathway. (A-F) Embryos injected with camk2g1 MO (0.25 ng), pkd2 MO (1 ng) or camk2g1 and pkd2 MOs (0.25 ng and 1 ng, respectively) were imaged at 72 hpf from lateral perspectives using DIC optics (A-C) or dorsal perspectives using immunofluorescence microscopy to identify α1 Na⁺/K⁺-ATPase (D-F). (G,H) Pronephric occlusions and convolution at 72 hpf (G; n=80-125) and pronephric migration as assessed by posterior ear-anterior kidney distance in µm at 72 hpf (H; n=60-82). (I,J) Cloacal cilia number (I) and cilia length (J) were determined at 24, 48 and 72 hpf. ^*P<0.005. (K-M) 72 hpf morphants immunolabeled with acetylated tubulin (green) and γ–tubulin (red) show a loss of cloacal cilia but retention of the basal body. Scale bars: 500 μm in A; 20 μm in D; 5 μm in K.

Expression Data

Antibody:	Ab1-atp1a1
Fish:	WT + MO1-camk2g1 + MO3-pkd2
Knockdown Reagents:	MO1-camk2g1 MO3-pkd2
Anatomical Term:	pronephros
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-camk2g1 + MO3-pkd2
Knockdown Reagents:	MO1-camk2g1 MO3-pkd2
Observed In:	cell migration involved in kidney development cloacal chamber ciliary basal body cloacal chamber cilium inner ear posterior-most region pronephric duct anterior-most region pronephric duct distal region pronephros anterior region
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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