FIGURE

Fig. S1

ID

ZDB-FIG-110909-79

Publication

Rothschild et al., 2011 - CaMK-II is a PKD2 target that promotes pronephric kidney development and stabilizes cilia

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Fig. S1

camk2g1 mRNA is expressed in the pronephric kidney. (A) Two alternative splice variants of the γ1 CaMK-II gene (γ1_C and γ1_F) were identified when cDNA from dissected kidneys or flow sorted kidney cells at 48 and 72 hpf was used as the template for RT-PCR. Alternative exon use in the variable domain is based on previous naming conventions. (B,B2) Whole-mount in situ hybridization was conducted using a γ1_C probe at 72 hpf and is shown in whole mounts and in cryosectioned embryos. The cloaca is indicated by an arrow in B and the pronephric duct by an arrow in B2. (C) FISH was performed on 48 hpf embryos with the γ1_C probe followed by immunostaining with acetylated tubulin to label pronephric ductal cilia. (D-F) Double fluorescent in situ hybridization demonstrates that γ1_C CaMK-II and the pronephric kidney marker pax2a are co-expressed in the distal pronephros.

Expression Data

Genes:	camk2g1 pax2a
Antibody:	Ab1-tuba
Fish:	WT
Anatomical Terms:	cloacal chamber pronephric duct pronephric duct cilium pronephric duct distal region
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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