FIGURE

Fig. 4

ID

ZDB-FIG-110531-18

Publication

Yeh et al., 2011 - Ptenb Mediates Gastrulation Cell Movements via Cdc42/AKT1 in Zebrafish

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Fig. 4

ptenb MOs specifically impairs gastrulation in a dose-dependent manner.

Knockdown of ptenb by MOs caused gastrulation defects in graded severity. Representative photographs of ptenb morphants with different severity are shown in (A) normal (B) intermediate and (C) severe. (D) Embryos were injected with StdMO, tMO₁, mis-tMO1 and tMO₂ at designated amount, incubated to 10 somite stage and the percentages of embryos with different severity are shown. (E) Embryos were injected with 15 ng of StdMO or tMO₁ in the absence or presence of ptenb mRNAs (50 pg), incubated and their degrees of gastrulation defect are shown. Each treatment was repeated at least 4 times.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO1-ptenb AB/TU + MO2-ptenb
Knockdown Reagents:	MO1-ptenb MO2-ptenb
Observed In:	gastrulation post-vent region whole organism
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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