FIGURE

Fig. 2

ID

ZDB-FIG-110121-8

Publication

Choudhry et al., 2011 - Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis

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Fig. 2

alcama morphants have cartilage defects similar to the edn1 class of mutants. Flatmounts of mandibular and hyoid cartilage from 5 dpf Alcian Blue-stained larvae (A, C, E, G); corresponding schematics (B, D, F, H). The joint between Meckel′s and palatoquadrate is fused in alcama morphants and furinA^-/- and edn1^-/- mutants. Similarly, the interhyal is absent in alcama morphants and furinA^-/- mutants leading to a fusion of the ceratohyal and hyosymplectic cartilages. alcama morphants and furinA^-/- mutants have misshapen Meckel′s cartilage and ceratohyal, but edn1^-/- mutants have the most severe defect with a lack of ceratohyal and severely misshapen Meckel′s cartilage. DV joint regions are indicated with arrows in A and B. Fusions at joints are indicated with asterisks in (C)–(H). Cartilages are labeled as followed: pq (palatoquadrate), mc (Meckel′s cartilage), hm (hyomandibula), ch (ceratohyal), sy (symplectic), and ih (interhyal). Scale bar: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	edn1^{tf216b/tf216b} furina^{td204e/td204e} WT + MO4-alcama
Knockdown Reagent:	MO4-alcama
Observed In:	ceratohyal cartilage hyosymplectic cartilage interhyal cartilage Meckel's cartilage palatoquadrate cartilage symplectic
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 349(2), Choudhry, P., Joshi, D., Funke, B., and Trede, N., Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis, 483-493, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.