FIGURE

Fig. 7

ID

ZDB-FIG-110121-13

Publication

Choudhry et al., 2011 - Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis

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Fig. 7

alcama and nadl1.1 interact during cartilage formation. (A) Lateral view of a 30 hpf WT larva stained with Zn-5 antibody (Fast Red) and anti-sense nadl1.1 RNA (Fluorescein), revealing that nadl1.1 is expressed in the NC adjacent to alcama in the endoderm. The bar graphs display the percentage of 30 hpf edn1^-/- (B) and WT (C) larvae with dlx5a staining after the indicated injections. (B) shows that nadl1.1 is required for alcama rescue of dlx5a expression in edn1^-/- mutants (p-value < 0.0001 by Fishers exact test). (C) and (D) show that alcama and nadl1.1 interact during dlx5a expression and cartilage morphogenesis. (D) Bar graph displaying the percentage of 5 dpf larvae with affected cartilage after the indicated injections (p-value < 0.0001 by χ² analysis). n indicates the number of experiments and N the total number of larvae. Scale bar: 50 μm.

Expression Data

Genes:	alcama l1camb
Antibody:	zn-5
Fish:	WT
Anatomical Terms:	head endoderm pharyngeal arch 1 neural crest pharyngeal arch 2 neural crest pharyngeal arch 3-7 neural crest
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 349(2), Choudhry, P., Joshi, D., Funke, B., and Trede, N., Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis, 483-493, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.