FIGURE

Fig. 6

ID

ZDB-FIG-110121-12

Publication

Choudhry et al., 2011 - Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis

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Fig. 6

nadl1.1 morphants phenocopy alcama morphant cartilage defects. Dorsal views (A–C) and lateral views (D, E) of 30 hpf larvae. nadl1.1 is expressed in the pharyngeal arches, diencephalon, telencephalon, hindbrain neurons, neural tube and pectoral fin in 30 hpf WT larvae (A). 30 hpf nadl 1.1 morphants have down-regulated hand2 (C) and dlx5a (E) expression as compared to uninjected WT (B, D). (F) Bar graph showing that the percentage of larvae with down-regulated (absent) dlx5a expression remains unchanged in the p53^-/- background (p-value = 1.000 by Fishers exact test, indicating that WT and p53^-/- mutants are not significantly different). Dissected mandibular and hyoid elements from 5 dpf WT and nadl1.1 morphant larvae stained with Alcian blue (G, H) and their corresponding schematics (G′, H′). The joint fusions in nadl1.1 morphants (H, H′) marked by * resemble those seen in alcama morphants. (I) Bar graph comparing the percentage of larvae with affected cartilage in alcama morphants in WT versus p53^-/- mutants (p-value = 0.1941 by Fishers exact test, indicating that WT and p53^-/- mutants are not significantly different). N is the total number of larvae from three experiments. Scale bars: 50 μm.

Expression Data

Genes:	dlx5a hand2 l1camb
Fish:	WT WT + MO2-l1camb
Knockdown Reagent:	MO2-l1camb
Anatomical Terms:	diencephalon head hindbrain neuron pectoral fin pharyngeal arch pharyngeal arch 1 pharyngeal arch 2 pharyngeal arch 3 pharyngeal arch 4 telencephalon
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-l1camb
Knockdown Reagent:	MO2-l1camb
Observed In:	ceratohyal cartilage hyosymplectic cartilage interhyal cartilage Meckel's cartilage palatoquadrate cartilage symplectic
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 349(2), Choudhry, P., Joshi, D., Funke, B., and Trede, N., Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis, 483-493, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.