FIGURE

Fig. 4

ID

ZDB-FIG-110113-4

Publication

Wang et al., 2011 - The Rho kinase Rock2b establishes anteroposterior asymmetry of the ciliated Kupffer's vesicle in zebrafish

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Fig. 4

Rock2b function in DFC/KV cells is required for normal asymmetric southpaw expression. (A-C) spaw is normally expressed in left LPM in control embryos (A) at 16- to 18-SS. In contrast, spaw expression was often bilateral in rock2b MO embryos (red arrows in B,C). shh expression (see arrow in A) indicated that embryonic midline structures were intact. (D) The frequency of altered spaw expression (right, bilateral or absent) in control embryos and embryos injected with rock2b MO or DNA encoding DN-Rock proteins. (E-G) spaw expression was altered in DFC^rock2b ^MO embryos (F,G), whereas normal left-sided expression was observed in most control DFC^{control MO} embryos (E). (H) The percentage of DFC^rock2b ^MO and control embryos with spaw defects. n=number of embryos analyzed.

Expression Data

Genes:	shha spaw
Fish:	AB AB + MO1-rock2b AB + MO2-rock2b
Knockdown Reagents:	MO1-rock2b MO2-rock2b
Anatomical Terms:	lateral plate mesoderm left side lateral plate mesoderm right side notochord
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-rock2b AB + MO2-rock2b
Knockdown Reagents:	MO1-rock2b MO2-rock2b
Observed In:	determination of left/right asymmetry in lateral mesoderm lateral plate mesoderm
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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