FIGURE

Fig. 3

ID

ZDB-FIG-101118-16

Publication

Shive et al., 2010 - brca2 in zebrafish ovarian development, spermatogenesis, and tumorigenesis

Other Figures

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Fig. 3

Germ cell specification, development, and survival in embryonic and adult brca2^Q658X homozygotes. (A) WISH with antisense (AS) and sense (S) RNA probes for vasa indicates appropriate migration and colonization of the gonadal ridge in WT, brca2^Q658X heterozygous, and brca2^Q658X homozygous embryos. (B) Testes from WT, brca2^Q658X heterozygotes, and brca2^Q658X homozygotes show spermatocysts containing meiotic spermatocytes. (C) TUNEL staining of testes from WT, brca2^Q658X heterozygotes, and brca2^Q658X homozygotes. Multiple clusters of apoptotic spermatocytes are present in testes from brca2^Q658X homozygotes (Inset). (D) Homozygous tp53^M214K mutation rescues ovarian development in brca2^Q658X homozygotes, but female brca2^Q658X homozygous;tp53^M214K homozygous zebrafish develop binucleate oocytes (Inset). sc, spermatocyst; msc, meiotic spermatocyst; I, type I oocyte; II, type II oocyte; III, type III oocyte. (Scale bars: 25 μm in B, 100 μm in C, and 500 μm in D.)

Expression Data

Gene:	ddx4
Fish:	WT brca2^hg5/hg5 brca2^hg5/+
Anatomical Term:	primordial germ cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	brca2^hg5/hg5 brca2^hg5/hg5; tp53^zdf1/zdf1
Observed In:	male meiotic nuclear division oocyte spermatocyte
Stage:	Adult

Phenotype Detail

Acknowledgments

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