ZFIN Image: Shive et al., 2010, Fig. 3

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Fig. 3

Germ cell specification, development, and survival in embryonic and adult brca2^Q658X homozygotes. (A) WISH with antisense (AS) and sense (S) RNA probes for vasa indicates appropriate migration and colonization of the gonadal ridge in WT, brca2^Q658X heterozygous, and brca2^Q658X homozygous embryos. (B) Testes from WT, brca2^Q658X heterozygotes, and brca2^Q658X homozygotes show spermatocysts containing meiotic spermatocytes. (C) TUNEL staining of testes from WT, brca2^Q658X heterozygotes, and brca2^Q658X homozygotes. Multiple clusters of apoptotic spermatocytes are present in testes from brca2^Q658X homozygotes (Inset). (D) Homozygous tp53^M214K mutation rescues ovarian development in brca2^Q658X homozygotes, but female brca2^Q658X homozygous;tp53^M214K homozygous zebrafish develop binucleate oocytes (Inset). sc, spermatocyst; msc, meiotic spermatocyst; I, type I oocyte; II, type II oocyte; III, type III oocyte. (Scale bars: 25 μm in B, 100 μm in C, and 500 μm in D.)

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA