ZFIN Figure: Bai et al., 2010, Fig. 5

Fig. 5

Effects of FACT, p-TEFb, and DSIF on Erythropoiesis

(A) Reduced erythropoiesis by inhibiting FACT and p-TEFb. In situ hybridization of βe3 was performed on 22 hpf zebrafish embryos treated with morpholino injection (ii and iii) or 35 μM flavopiridol (iv). See also Figure S4.

(B) Flavopiridol treatment blocks erythroid differentiation of human CD34⁺ cells. Cells were harvested at day 11 after differentiation (close to terminal differentiation stage), stained for benzidine (brown), and counterstained with May-Grunwald (blue).

(C) Similar morphology of sun mutants and spt5 morphants at 36 hpf.

(D) Rescue of mon mutants by spt5 morpholino and mutants, shown by in situ hybridization of βe3 globin at 22 hpf. Note the rescue efficiency was 100% in each case (ii–iv).

Expression Data

Gene:	hbbe3
Fish:	WT trim33^tg234/tg234 WT + MO3-cdk9 trim33^tg234/tg234 + MO1-supt5h WT + MO1-ssrp1a + MO1-supt16h supt5h^m806/m806; trim33^tg234/tg234 trim33^tg234/tg234; Df(Chr15:supt5h)s30/s30
Knockdown Reagents:	MO1-ssrp1a MO1-supt5h MO1-supt16h MO3-cdk9
Anatomical Term:	intermediate cell mass of mesoderm
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdc73^cz400/cz400 trim33^tg234/tg234 WT + MO1-supt5h WT + MO3-cdk9 trim33^tg234/tg234 + MO1-supt5h WT + MO1-ssrp1a + MO1-supt16h supt5h^m806/m806; trim33^tg234/tg234 trim33^tg234/tg234; Df(Chr15:supt5h)s30/s30
Knockdown Reagents:	MO1-ssrp1a MO1-supt5h MO1-supt16h MO3-cdk9
Observed In:	erythrocyte differentiation heart melanocyte post-vent region
Stage Range:	26+ somites to Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 142(1), Bai, X., Kim, J., Yang, Z., Jurynec, M.J., Akie, T.E., Lee, J., LeBlanc, J., Sessa, A., Jiang, H., DiBiase, A., Zhou, Y., Grunwald, D.J., Lin, S., Cantor, A.B., Orkin, S.H., and Zon, L.I., TIF1gamma controls erythroid cell fate by regulating transcription elongation, 133-143, Copyright (2010) with permission from Elsevier. Full text @ Cell