ZFIN Image: Bai et al., 2010, Fig. 5

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Figure Caption

Fig. 5 Effects of FACT, p-TEFb, and DSIF on Erythropoiesis

(A) Reduced erythropoiesis by inhibiting FACT and p-TEFb. In situ hybridization of βe3 was performed on 22 hpf zebrafish embryos treated with morpholino injection (ii and iii) or 35 μM flavopiridol (iv). See also Figure S4.

(B) Flavopiridol treatment blocks erythroid differentiation of human CD34⁺ cells. Cells were harvested at day 11 after differentiation (close to terminal differentiation stage), stained for benzidine (brown), and counterstained with May-Grunwald (blue).

(C) Similar morphology of sun mutants and spt5 morphants at 36 hpf.

(D) Rescue of mon mutants by spt5 morpholino and mutants, shown by in situ hybridization of βe3 globin at 22 hpf. Note the rescue efficiency was 100% in each case (ii–iv).

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Acknowledgments

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Reprinted from Cell, 142(1), Bai, X., Kim, J., Yang, Z., Jurynec, M.J., Akie, T.E., Lee, J., LeBlanc, J., Sessa, A., Jiang, H., DiBiase, A., Zhou, Y., Grunwald, D.J., Lin, S., Cantor, A.B., Orkin, S.H., and Zon, L.I., TIF1gamma controls erythroid cell fate by regulating transcription elongation, 133-143, Copyright (2010) with permission from Elsevier. Full text @ Cell