FIGURE

Fig. 1

ID

ZDB-FIG-100723-6

Publication

Bai et al., 2010 - TIF1gamma controls erythroid cell fate by regulating transcription elongation

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Fig. 1

Genetic Suppressor Screen in the mon Mutant

(A) Scheme of generating viable mon transgenic fish using a BAC transgene.

(B) Scheme of the suppressor screen. BAC transgenic fish are green fluorescent. In F1 generation, three groups of embryos were obtained: transgene homozygous (mon; Tg/Tg), transgene heterozygous (mon; Tg/+), and embryos with no transgene (mon). Only transgene heterozygous (in the red circle) were raised up to adults. Double in situ hybridization of GFP and βe3 globin was performed on F2 haploid embryos. Note the GFP staining on mon; Tg haploids (strong in the head and weak throughout the body) but not on haploids lacking the transgene. “sup” indicates a suppressor mutation.

(D) In situ hybridization of βe3 globin at 22 hpf.

See also Figure S1.

Expression Data

Gene:	hbbe3
Fish:	WT cdc73^cz400/cz400 trim33^tg234/tg234 cdc73^cz400/cz400; trim33^tg234/tg234
Anatomical Term:	intermediate cell mass of mesoderm
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdc73^cz400/cz400 cdc73^cz400/cz400 trim33^tg234/tg234 cdc73^cz400/cz400; trim33^tg234/tg234
Observed In:	erythrocyte differentiation heart melanocyte
Stage Range:	26+ somites to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 142(1), Bai, X., Kim, J., Yang, Z., Jurynec, M.J., Akie, T.E., Lee, J., LeBlanc, J., Sessa, A., Jiang, H., DiBiase, A., Zhou, Y., Grunwald, D.J., Lin, S., Cantor, A.B., Orkin, S.H., and Zon, L.I., TIF1gamma controls erythroid cell fate by regulating transcription elongation, 133-143, Copyright (2010) with permission from Elsevier. Full text @ Cell