FIGURE

Fig. 3

ID

ZDB-FIG-091109-21

Publication

Zeng et al., 2009 - miR-145 directs intestinal maturation in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Normalization of gata6 levels rescues loss of miR-145. (A–C) Knockdown of miR-145 results in increased expression of gata6 in zebrafish gut at 48 hpf. (D–F) Overexpression of miR-145 leads to decreased gata6 expression in the gut of 96 hpf embryos. Pericardial edema (arrowhead) is also found in miR-145 morphant. (G–I) gata6 knockdown normalizes the gut morphology (arrow) of miR-145 morphants. (J) qPCR shows strong up-regulation of gata6 in miR-145 morphants of 48 hpf. (K) qPCR indicates significantly decreased expression of gata6 in 48 hpf miR-145 mimic treated embryos. Arrows, gut; bars, mean ± SEM. *, P < 0.01. (L) The number of embryos exhibiting normal gut morphology in the rescue was analyzed by using Student′s t test (P < 0.05, n = 286). (Scale bar, 200 μm.)

Expression Data

Gene:	gata6
Fish:	WT WT + MO1-mir145
Knockdown Reagent:	MO1-mir145
Anatomical Term:	gut
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mir145 WT + MO1-gata6 + MO1-mir145
Knockdown Reagents:	MO1-gata6 MO1-mir145
Observed In:	gut
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA