FIGURE

Fig. S1

ID

ZDB-FIG-091106-4

Publication

Zeng et al., 2009 - miR-145 directs intestinal maturation in zebrafish

Other Figures

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Fig. S1

Phenotype after injection of miR-145 morpholino and mimic. (A) LNA in situ hybridization shows ubiquitous expression of miR-145 in uninjected wild-type embryos (UIC) and B) control MO-injected embryos. (C) miR-145 expression is significantly reduced by miR-145 MO and (D) pre-miR-145 morpholino. (E) Expression of miR-145 in uninjected or (F) control mimic expressing embryos is low in comparison to (G) miR-145 mimic-injected embryos. (H) qPCR indicates significant knocking down of miR-145 in 24 hpf embryos treated with mature miR-145 and pre-miR-145 morpholino. (I–K) Co-injection of miR-145 mimic with miR-145 morpholino can partially rescue the phenotype of miR-145 morphants. (L) pre-miR-145 MO induces a similar phenotype to miR-145 MO with gut morphological defects (arrow) and pericardial edema (arrowhead) in 96 hpf embryos. Bars, mean ± SEM. *, P < 0.05. (Scale bars, 200 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mir145
Knockdown Reagent:	MO1-mir145
Observed In:	gut pericardium
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA